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<œÚ9N•©ÒæêJ: _”è¢ÎávOÅ2	kq| fc-x5¦b %m‰¢a¯ùýÛù³¹¿Åëp	P
h
‡
“
„AjŸfÃ:% ÉªEEi„�»�Ïó)39úq	*ip…ðƒ™—û×ã’ŸGÃ¬! V R³X~”9o/Ah(òZ~çC^€¹üEQ¢ºÅ×s  ô9ÌH™EHH	y	]
r
ð
àéK +ó!@Udh�	ã ßç]ŽðÞ• ƒˆè½¿
Å
%L©Wïÿ×�’­æ$  Nhw¶à!OƒW
l
-e8s”¾ßø3OW1€GP|{� & $^lˆÈ®øDuüƒ7N8ùÄÐ	Ù	
!
g
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q
KP¡¬Äæ,  fgñò­÷µMø‚ÃÏ	Ø	
 
[
p
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:d½÷þMNOŸ DEFz§ÀÁÿ  ,š  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                             -                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             	                                                                                                                                                                                                                                                       "                
                                                                                                                                    	                                                 
               )   !   
         
                                                      	                                                ;             %                  l   F                     R                                                                                                                                                                        -                                                                                                                                                                                                                                                                                                     	                                                                                           "      
                                              	                 
     ) ! 
   
                  	                ;     %      l F       R   hû  iÿ  j  jC  n  rM  x	gàA$xyG	Ž
fœ½Úc#Éàvï…6á	)
	J œ2¤	Î:­SMÇo	Ý`í¸
	onë	3
@¾ MÇçkƒ*fsŸ¨£¶Î7Rpo	ë	3
f
µ
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S¯Ý
<`iÍ@Izû'{¾0ií}ž»vÀgvvbï…6·syZÑ	
Zz¡±ä"	ün	sosƒz~~< z Å4~Öƒ/A² †‘~
Bç�èˆá";íæÁíæ`	í
Tr§ú´5'„‹|WYÊø/¹Ú]Û/ZGPËŒ0¼Â\Ñ¡m	&>€Ë`ÿ~ ™™	4YL©%r§ú´5Ú]‹'wÆ““ã ÏÎø  -2¸;Á ;„ÿ \¦	V&ð
c~ ùzsÜ0;m	€Ÿ-Äè—¶pˆ‰®Ú]¤²ð	8
ôÒÆá7|
¨j
ÉlCKÚ]¯ƒõ ÊŸø/˜¤¹Ô	
Ú4]ozÛ/ZFG¥Î¡ï|lèíØÐqkƒçêqë¾Kàañ} 8o'Ð	
“
¶
ð
;<Þkœ½×ü¡R¢ÀÏÎ|Ïbž±gÏkÎyeÉËÒäÝ
ËÙ	!
¶ä1¡ådËªRêC�a“
Í  -ùzs2Äè—¶p¸Ü0;ÁšÎ¾„a“
ð
k¤‡�w Mz~NoŸãêý®²@NyÝ	ð	%
8
<rÒÞRÉ0Mº5béa“
Þ²ð	8
K ŸÈãŠàììýÏ ?VZì£FN, Þ×óºÖó×Ë<c
x
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IÛ-YL|lèí©ØÐ%úÛ	#
™gi™ò	:
âç ‹¨ ç Àì=¾Ý	%	„‹;   Í �ŠÅsÑ
|ê‡=êpÒ4„ÎÑ¾  ã ¿wa 	yÉÝâÞOUJôµ
(ÍöŠ„âO‹„“kqƒç�Æxš k€5N	©á¯D) Ò"Cv€šY5|#~	� Ÿ‚ãàì  ÈÉzÔ8‹í½þR~î9o(Ñ	

<Z~œçCü•¢¦Ž»që¾Ç sÄàç,šE­Q¢´W˜›f®Ø L�eè?íh9¥ôãñ8¾×WÂ|1�e×$<½ÖUFî°¶¼Â“æÉ7eí9ãÄàç,E­˜›fÇ sšQ¢´W®ýù³¹¿ëh
„Ÿ:ÙNKœe³Ý·ØçA²; Ç s®Äàç{,»
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<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
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<LastModified UTC="7:6:47">2024.8.21</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:51:12">2019.6.4</Created>
<LastModified UTC="11:54:3">2019.6.13</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<UUID>bef72f78-a4ea-4aa3-b79c-32d55cec59e2</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.29</Created>
<LastModified UTC="6:50:25">2019.6.2</LastModified>
<CreatedBy>æµ·å�‰æµ©æ ¼</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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boundStrand="1" annealedBases="TCGGGTTTCGCCACCTCTG" meltingTemperature="61"><Component hybridizedRange="3460-3478" bases="TCGGGTTTCGCCACCTCTG"/></BindingSite><BindingSite simplified="1" location="3460-3478" boundStrand="1" annealedBases="TCGGGTTTCGCCACCTCTG" meltingTemperature="61"><Component hybridizedRange="3460-3478" bases="TCGGGTTTCGCCACCTCTG"/></BindingSite></Primer><Primer recentID="15" name="HH-L-cexuF" sequence="ACGACAGGTTTCCCGACTG"><BindingSite location="3148-3166" boundStrand="1" annealedBases="ACGACAGGTTTCCCGACTG" meltingTemperature="59"><Component hybridizedRange="3148-3166" bases="ACGACAGGTTTCCCGACTG"/></BindingSite><BindingSite simplified="1" location="3148-3166" boundStrand="1" annealedBases="ACGACAGGTTTCCCGACTG" meltingTemperature="59"><Component hybridizedRange="3148-3166" bases="ACGACAGGTTTCCCGACTG"/></BindingSite></Primer><Primer recentID="12" name="PQE-F" sequence="TGAGCGGATAACAATTTCAC"><BindingSite location="3024-3043" boundStrand="1" annealedBases="TGAGCGGATAACAATTTCAC" meltingTemperature="52"><Component hybridizedRange="3024-3043" bases="TGAGCGGATAACAATTTCAC"/></BindingSite><BindingSite simplified="1" location="3024-3043" boundStrand="1" annealedBases="TGAGCGGATAACAATTTCAC" meltingTemperature="52"><Component hybridizedRange="3024-3043" bases="TGAGCGGATAACAATTTCAC"/></BindingSite></Primer><Primer recentID="11" name="PCMV-R" sequence="TCCAAACTCATCAATGTATC"><BindingSite location="2935-2954" boundStrand="0" annealedBases="TCCAAACTCATCAATGTATC" meltingTemperature="49"><Component hybridizedRange="2935-2954" bases="TCCAAACTCATCAATGTATC"/></BindingSite><BindingSite simplified="1" location="2935-2954" boundStrand="0" annealedBases="TCCAAACTCATCAATGTATC" meltingTemperature="49"><Component hybridizedRange="2935-2954" bases="TCCAAACTCATCAATGTATC"/></BindingSite></Primer><Primer recentID="10" name="PIRES-R" sequence="CACTGCATTCTAGTTGTGGT"><BindingSite location="2912-2931" boundStrand="0" annealedBases="CACTGCATTCTAGTTGTGGT" meltingTemperature="56"><Component hybridizedRange="2912-2931" bases="CACTGCATTCTAGTTGTGGT"/></BindingSite><BindingSite simplified="1" location="2912-2931" boundStrand="0" annealedBases="CACTGCATTCTAGTTGTGGT" meltingTemperature="56"><Component hybridizedRange="2912-2931" bases="CACTGCATTCTAGTTGTGGT"/></BindingSite></Primer><Primer recentID="9" name="SV40pA-R" sequence="GAAATTTGTGATGCTATTGC"><BindingSite location="2873-2892" boundStrand="1" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="51"><Component hybridizedRange="2873-2892" bases="GAAATTTGTGATGCTATTGC"/></BindingSite><BindingSite simplified="1" location="2873-2892" boundStrand="1" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="51"><Component hybridizedRange="2873-2892" bases="GAAATTTGTGATGCTATTGC"/></BindingSite></Primer><Primer recentID="5" name="EBV-Reverse" sequence="CAGCTTATAATGGTTACAAAT"><BindingSite location="2849-2869" boundStrand="0" annealedBases="CAGCTTATAATGGTTACAAAT" meltingTemperature="48"><Component hybridizedRange="2849-2869" bases="CAGCTTATAATGGTTACAAAT"/></BindingSite><BindingSite simplified="1" location="2849-2869" boundStrand="0" annealedBases="CAGCTTATAATGGTTACAAAT" meltingTemperature="48"><Component hybridizedRange="2849-2869" bases="CAGCTTATAATGGTTACAAAT"/></BindingSite></Primer><Primer recentID="3" name="CMV-Forward" sequence="CGCAAATGGGCGGTAGGCGTG"><BindingSite location="768-788" boundStrand="0" annealedBases="CGCAAATGGGCGGTAGGCGTG" meltingTemperature="65"><Component hybridizedRange="768-788" bases="CGCAAATGGGCGGTAGGCGTG"/></BindingSite><BindingSite simplified="1" location="768-788" boundStrand="0" annealedBases="CGCAAATGGGCGGTAGGCGTG" meltingTemperature="65"><Component hybridizedRange="768-788" bases="CGCAAATGGGCGGTAGGCGTG"/></BindingSite></Primer><Primer recentID="2" name="PEGFP-N-5" sequence="TGGGAGGTCTATATAAGCAGAG"><BindingSite location="794-815" boundStrand="0" annealedBases="TGGGAGGTCTATATAAGCAGAG" meltingTemperature="54"><Component hybridizedRange="794-815" bases="TGGGAGGTCTATATAAGCAGAG"/></BindingSite><BindingSite simplified="1" location="794-815" boundStrand="0" annealedBases="TGGGAGGTCTATATAAGCAGAG" meltingTemperature="54"><Component hybridizedRange="794-815" bases="TGGGAGGTCTATATAAGCAGAG"/></BindingSite></Primer><Primer recentID="0" name="M13R(-48)" sequence="AGCGGATAACAATTTCACACAGGA"><BindingSite location="3018-3041" boundStrand="1" annealedBases="AGCGGATAACAATTTCACACAGGA" meltingTemperature="58"><Component hybridizedRange="3018-3041" bases="AGCGGATAACAATTTCACACAGGA"/></BindingSite><BindingSite simplified="1" location="3018-3041" boundStrand="1" annealedBases="AGCGGATAACAATTTCACACAGGA" meltingTemperature="58"><Component hybridizedRange="3018-3041" bases="AGCGGATAACAATTTCACACAGGA"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>bef72f78-a4ea-4aa3-b79c-32d55cec59e2</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.29</Created>
<LastModified UTC="14:0:49">2019.5.5</LastModified>
<CreatedBy>æµ·å�‰æµ©æ ¼</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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  ö<?xml version="1.0"?><Features nextValidID="16"><Feature recentID="15" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3031-3047" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3007-3023" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="863-881" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3100-3121" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3055-3061" color="#ffffff" type="standard"/><Segment range="3062-3079" color="#ffffff" type="standard"/><Segment name="-35" range="3080-3085" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5026-5130" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2837-2958" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1920-2055" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="615-818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1028-1252" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1740-2069" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-614" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1298-1726" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="2" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3409-3994" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4956" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4165-4956" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="4957-5025" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2078-2677" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  ÷<?xml version="1.0"?><Primers recycledIDs="21,19,1,4,14,18,7,13,20,6" nextValidID="23"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="8" name="Primer 1" sequence="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2077-2326" boundStrand="0" annealedBases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgaccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag" meltingTemperature="90"><Component hybridizedRange="2077-2127" bases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccc"/><Component bases="a"/><Component hybridizedRange="2129-2189" bases="gggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcga"/><Component bases="t"/><Component hybridizedRange="2191-2326" bases="ccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag"/></BindingSite><BindingSite simplified="1" location="2077-2326" boundStrand="0" annealedBases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgaccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag" meltingTemperature="90"><Component hybridizedRange="2077-2127" bases="atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccc"/><Component bases="a"/><Component hybridizedRange="2129-2189" bases="gggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcga"/><Component bases="t"/><Component hybridizedRange="2191-2326" bases="ccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag"/></BindingSite></Primer><Primer recentID="22" name="M13R" sequence="CAGGAAACAGCTATGAC"><BindingSite location="3006-3022" boundStrand="1" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="3006-3022" bases="CAGGAAACAGCTATGAC"/></BindingSite><BindingSite simplified="1" location="3006-3022" boundStrand="1" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="3006-3022" bases="CAGGAAACAGCTATGAC"/></BindingSite></Primer><Primer recentID="17" name="V1_5" sequence="GGACTTTCCAAAATGTCG"><BindingSite location="730-747" boundStrand="0" annealedBases="GGACTTTCCAAAATGTCG" meltingTemperature="51"><Component hybridizedRange="730-747" bases="GGACTTTCCAAAATGTCG"/></BindingSite><BindingSite simplified="1" location="730-747" boundStrand="0" annealedBases="GGACTTTCCAAAATGTCG" meltingTemperature="51"><Component hybridizedRange="730-747" bases="GGACTTTCCAAAATGTCG"/></BindingSite></Primer><Primer recentID="16" name="HH-pBRori-cexuR" sequence="TCGGGTTTCGCCACCTCTG"><BindingSite location="3460-3478" boundStrand="1" annealedBases="TCGGGTTTCGCCACCTCTG" meltingTemperature="61"><Component hybridizedRange="3460-3478" bases="TCGGGTTTCGCCACCTCTG"/></BindingSite><BindingSite simplified="1" location="3460-3478" boundStrand="1" annealedBases="TCGGGTTTCGCCACCTCTG" meltingTemperature="61"><Component hybridizedRange="3460-3478" bases="TCGGGTTTCGCCACCTCTG"/></BindingSite></Primer><Primer recentID="15" name="HH-L-cexuF" sequence="ACGACAGGTTTCCCGACTG"><BindingSite location="3148-3166" boundStrand="1" annealedBases="ACGACAGGTTTCCCGACTG" meltingTemperature="59"><Component hybridizedRange="3148-3166" bases="ACGACAGGTTTCCCGACTG"/></BindingSite><BindingSite simplified="1" location="3148-3166" boundStrand="1" annealedBases="ACGACAGGTTTCCCGACTG" meltingTemperature="59"><Component hybridizedRange="3148-3166" bases="ACGACAGGTTTCCCGACTG"/></BindingSite></Primer><Primer recentID="12" name="PQE-F" sequence="TGAGCGGATAACAATTTCAC"><BindingSite location="3024-3043" boundStrand="1" annealedBases="TGAGCGGATAACAATTTCAC" meltingTemperature="52"><Component hybridizedRange="3024-3043" bases="TGAGCGGATAACAATTTCAC"/></BindingSite><BindingSite simplified="1" location="3024-3043" boundStrand="1" annealedBases="TGAGCGGATAACAATTTCAC" meltingTemperature="52"><Component hybridizedRange="3024-3043" bases="TGAGCGGATAACAATTTCAC"/></BindingSite></Primer><Primer recentID="11" name="PCMV-R" sequence="TCCAAACTCATCAATGTATC"><BindingSite location="2935-2954" boundStrand="0" annealedBases="TCCAAACTCATCAATGTATC" meltingTemperature="49"><Component hybridizedRange="2935-2954" bases="TCCAAACTCATCAATGTATC"/></BindingSite><BindingSite simplified="1" location="2935-2954" boundStrand="0" annealedBases="TCCAAACTCATCAATGTATC" meltingTemperature="49"><Component hybridizedRange="2935-2954" bases="TCCAAACTCATCAATGTATC"/></BindingSite></Primer><Primer recentID="10" name="PIRES-R" sequence="CACTGCATTCTAGTTGTGGT"><BindingSite location="2912-2931" boundStrand="0" annealedBases="CACTGCATTCTAGTTGTGGT" meltingTemperature="56"><Component hybridizedRange="2912-2931" bases="CACTGCATTCTAGTTGTGGT"/></BindingSite><BindingSite simplified="1" location="2912-2931" boundStrand="0" annealedBases="CACTGCATTCTAGTTGTGGT" meltingTemperature="56"><Component hybridizedRange="2912-2931" bases="CACTGCATTCTAGTTGTGGT"/></BindingSite></Primer><Primer recentID="9" name="SV40pA-R" sequence="GAAATTTGTGATGCTATTGC"><BindingSite location="2873-2892" boundStrand="1" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="51"><Component hybridizedRange="2873-2892" bases="GAAATTTGTGATGCTATTGC"/></BindingSite><BindingSite simplified="1" location="2873-2892" boundStrand="1" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="51"><Component hybridizedRange="2873-2892" 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<UUID>bef72f78-a4ea-4aa3-b79c-32d55cec59e2</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
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<LastModified UTC="14:0:38">2019.5.5</LastModified>
<CreatedBy>æµ·å�‰æµ©æ ¼</CreatedBy>
<SequenceClass>UNA</SequenceClass>
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<UUID>bef72f78-a4ea-4aa3-b79c-32d55cec59e2</UUID>
<Type>Synthetic</Type>
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<LastModified UTC="13:37:25">2019.5.5</LastModified>
<CreatedBy>æµ·å�‰æµ©æ ¼</CreatedBy>
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<LastModified UTC="2:42:7">2019.6.1</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:20:38">2019.5.12</Created>
<LastModified UTC="3:49:10">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<UUID>e6875514-1d5c-48a6-93f8-ffb14f8af1fe</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:20:38">2019.5.12</Created>
<LastModified UTC="3:49:5">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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  f<Notes>
<UUID>e6875514-1d5c-48a6-93f8-ffb14f8af1fe</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:20:38">2019.5.12</Created>
<LastModified UTC="3:44:14">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<LastModified UTC="3:40:28">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<LastModified UTC="3:36:6">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:20:38">2019.5.12</Created>
<LastModified UTC="3:30:42">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<LastModified UTC="3:28:32">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<UUID>e6875514-1d5c-48a6-93f8-ffb14f8af1fe</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:20:38">2019.5.12</Created>
<LastModified UTC="3:26:42">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<LastModified UTC="3:24:48">2019.5.12</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<CreatedBy>Triple Threat</CreatedBy>
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  Y     K         
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